Embelin Inhibits Invasion and Migration of MDA‐MB‐231 Breast Cancer Cells by Suppression of CXC Chemokine Receptor 4, Matrix Metalloproteinases‐9/2, and Epithelial–Mesenchymal Transition
Identifieur interne : 000E18 ( Main/Exploration ); précédent : 000E17; suivant : 000E19Embelin Inhibits Invasion and Migration of MDA‐MB‐231 Breast Cancer Cells by Suppression of CXC Chemokine Receptor 4, Matrix Metalloproteinases‐9/2, and Epithelial–Mesenchymal Transition
Auteurs : Hanwool Lee [Corée du Sud] ; Jeong-Hyeon Ko [Corée du Sud] ; Seung Ho Baek [Corée du Sud] ; Dongwoo Nam [Corée du Sud] ; Seok Geun Lee [Corée du Sud] ; Junhee Lee [Corée du Sud] ; Woong Mo Yang [Corée du Sud] ; Jae-Young Um [Corée du Sud] ; Sung-Hoon Kim [Corée du Sud] ; Bum Sang Shim [Corée du Sud] ; Kwang Seok Ahn [Corée du Sud]Source :
- Phytotherapy Research [ 0951-418X ] ; 2016-06.
Abstract
Embelin (EB) is a benzoquinone derivative isolated from Embelia ribes Burm plant. Recent scientific evidence shows that EB induces apoptosis and inhibits migration and invasion in highly metastatic human breast cancer cells. However, the exact mechanisms of EB in tumor metastasis and invasion have not been fully elucidated. Here, we investigated the underlying mechanisms of antimetastatic activities of EB in breast cancer cells. The EB downregulated the chemokine receptor 4 (CXCR4) as well as matrix metalloproteinase (MMP)‐9/2 expression and upregulated the tissue inhibitor of metalloproteinase 1 expression in MDA‐MB‐231 cells under noncytotoxic concentrations but not in MCF‐7 cells. Additionally, EB inhibited the CXC motif chemokine ligand 12 induced invasion and migration activities of MDA‐MB‐231 cells. A detailed study of underlying mechanisms revealed that the regulation of the downregulation of CXCR4 was at the transcriptional level, as indicated by the downregulation of mRNA expression and suppression of nuclear factor‐kappa B (NF‐κB) activation. It further reduced the binding of NF‐κB to the CXCR4 promoter. Besides, EB downregulated mesenchymal marker proteins (neural cadherin and vimentin) and concurrently upregulated epithelial markers (epithelial cadherin and occludin). Overall, these findings suggest that EB can abrogate breast cancer cell invasion and metastasis by suppression of CXCR4, MMP‐9/2 expressions, and inhibition of epithelial–mesenchymal transition and thus may have a great potential to suppress metastasis of breast cancer. Copyright © 2016 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/ptr.5612
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001A26
- to stream Istex, to step Curation: 001A26
- to stream Istex, to step Checkpoint: 000037
- to stream Main, to step Merge: 000E19
- to stream Main, to step Curation: 000E18
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Embelin Inhibits Invasion and Migration of MDA‐MB‐231 Breast Cancer Cells by Suppression of CXC Chemokine Receptor 4, Matrix Metalloproteinases‐9/2, and Epithelial–Mesenchymal Transition</title><author><name sortKey="Lee, Hanwool" sort="Lee, Hanwool" uniqKey="Lee H" first="Hanwool" last="Lee">Hanwool Lee</name></author><author><name sortKey="Ko, Jeong Yeon" sort="Ko, Jeong Yeon" uniqKey="Ko J" first="Jeong-Hyeon" last="Ko">Jeong-Hyeon Ko</name></author><author><name sortKey="Baek, Seung Ho" sort="Baek, Seung Ho" uniqKey="Baek S" first="Seung Ho" last="Baek">Seung Ho Baek</name></author><author><name sortKey="Nam, Dongwoo" sort="Nam, Dongwoo" uniqKey="Nam D" first="Dongwoo" last="Nam">Dongwoo Nam</name></author><author><name sortKey="Lee, Seok Geun" sort="Lee, Seok Geun" uniqKey="Lee S" first="Seok Geun" last="Lee">Seok Geun Lee</name></author><author><name sortKey="Lee, Junhee" sort="Lee, Junhee" uniqKey="Lee J" first="Junhee" last="Lee">Junhee Lee</name></author><author><name sortKey="Yang, Woong Mo" sort="Yang, Woong Mo" uniqKey="Yang W" first="Woong Mo" last="Yang">Woong Mo Yang</name></author><author><name sortKey="Um, Jae Oung" sort="Um, Jae Oung" uniqKey="Um J" first="Jae-Young" last="Um">Jae-Young Um</name></author><author><name sortKey="Kim, Sung Oon" sort="Kim, Sung Oon" uniqKey="Kim S" first="Sung-Hoon" last="Kim">Sung-Hoon Kim</name></author><author><name sortKey="Shim, Bum Sang" sort="Shim, Bum Sang" uniqKey="Shim B" first="Bum Sang" last="Shim">Bum Sang Shim</name></author><author><name sortKey="Ahn, Kwang Seok" sort="Ahn, Kwang Seok" uniqKey="Ahn K" first="Kwang Seok" last="Ahn">Kwang Seok Ahn</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0DEE67EBFC190620F5F6DF24DDF6DAA58D7DD1F6</idno><date when="2016" year="2016">2016</date><idno type="doi">10.1002/ptr.5612</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-JRQ20Z8Z-V/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001A26</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001A26</idno><idno type="wicri:Area/Istex/Curation">001A26</idno><idno type="wicri:Area/Istex/Checkpoint">000037</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000037</idno><idno type="wicri:doubleKey">0951-418X:2016:Lee H:embelin:inhibits:invasion</idno><idno type="wicri:Area/Main/Merge">000E19</idno><idno type="wicri:Area/Main/Curation">000E18</idno><idno type="wicri:Area/Main/Exploration">000E18</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Embelin Inhibits Invasion and Migration of MDA‐MB‐231 Breast Cancer Cells by Suppression of CXC Chemokine Receptor 4, Matrix Metalloproteinases‐9/2, and Epithelial–Mesenchymal Transition</title><author><name sortKey="Lee, Hanwool" sort="Lee, Hanwool" uniqKey="Lee H" first="Hanwool" last="Lee">Hanwool Lee</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Cancer Preventive Material Development, Graduate School, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Ko, Jeong Yeon" sort="Ko, Jeong Yeon" uniqKey="Ko J" first="Jeong-Hyeon" last="Ko">Jeong-Hyeon Ko</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Cancer Preventive Material Development, Graduate School, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Baek, Seung Ho" sort="Baek, Seung Ho" uniqKey="Baek S" first="Seung Ho" last="Baek">Seung Ho Baek</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Nam, Dongwoo" sort="Nam, Dongwoo" uniqKey="Nam D" first="Dongwoo" last="Nam">Dongwoo Nam</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Seok Geun" sort="Lee, Seok Geun" uniqKey="Lee S" first="Seok Geun" last="Lee">Seok Geun Lee</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Junhee" sort="Lee, Junhee" uniqKey="Lee J" first="Junhee" last="Lee">Junhee Lee</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Woong Mo" sort="Yang, Woong Mo" uniqKey="Yang W" first="Woong Mo" last="Yang">Woong Mo Yang</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Um, Jae Oung" sort="Um, Jae Oung" uniqKey="Um J" first="Jae-Young" last="Um">Jae-Young Um</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Sung Oon" sort="Kim, Sung Oon" uniqKey="Kim S" first="Sung-Hoon" last="Kim">Sung-Hoon Kim</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Shim, Bum Sang" sort="Shim, Bum Sang" uniqKey="Shim B" first="Bum Sang" last="Shim">Bum Sang Shim</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation></author><author><name sortKey="Ahn, Kwang Seok" sort="Ahn, Kwang Seok" uniqKey="Ahn K" first="Kwang Seok" last="Ahn">Kwang Seok Ahn</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Korean Medicine, Kyung Hee University, 24 Kyungheedae‐ro, Seoul 130‐701, Dongdaemun‐gu</wicri:regionArea><wicri:noRegion>Dongdaemun‐gu</wicri:noRegion></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Corée du Sud</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Phytotherapy Research</title><title level="j" type="alt">PHYTOTHERAPY RESEARCH</title><idno type="ISSN">0951-418X</idno><idno type="eISSN">1099-1573</idno><imprint><biblScope unit="vol">30</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1021">1021</biblScope><biblScope unit="page" to="1032">1032</biblScope><biblScope unit="page-count">12</biblScope><date type="published" when="2016-06">2016-06</date></imprint><idno type="ISSN">0951-418X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0951-418X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Embelin (EB) is a benzoquinone derivative isolated from Embelia ribes Burm plant. Recent scientific evidence shows that EB induces apoptosis and inhibits migration and invasion in highly metastatic human breast cancer cells. However, the exact mechanisms of EB in tumor metastasis and invasion have not been fully elucidated. Here, we investigated the underlying mechanisms of antimetastatic activities of EB in breast cancer cells. The EB downregulated the chemokine receptor 4 (CXCR4) as well as matrix metalloproteinase (MMP)‐9/2 expression and upregulated the tissue inhibitor of metalloproteinase 1 expression in MDA‐MB‐231 cells under noncytotoxic concentrations but not in MCF‐7 cells. Additionally, EB inhibited the CXC motif chemokine ligand 12 induced invasion and migration activities of MDA‐MB‐231 cells. A detailed study of underlying mechanisms revealed that the regulation of the downregulation of CXCR4 was at the transcriptional level, as indicated by the downregulation of mRNA expression and suppression of nuclear factor‐kappa B (NF‐κB) activation. It further reduced the binding of NF‐κB to the CXCR4 promoter. Besides, EB downregulated mesenchymal marker proteins (neural cadherin and vimentin) and concurrently upregulated epithelial markers (epithelial cadherin and occludin). Overall, these findings suggest that EB can abrogate breast cancer cell invasion and metastasis by suppression of CXCR4, MMP‐9/2 expressions, and inhibition of epithelial–mesenchymal transition and thus may have a great potential to suppress metastasis of breast cancer. Copyright © 2016 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Lee, Hanwool" sort="Lee, Hanwool" uniqKey="Lee H" first="Hanwool" last="Lee">Hanwool Lee</name></noRegion><name sortKey="Ahn, Kwang Seok" sort="Ahn, Kwang Seok" uniqKey="Ahn K" first="Kwang Seok" last="Ahn">Kwang Seok Ahn</name><name sortKey="Ahn, Kwang Seok" sort="Ahn, Kwang Seok" uniqKey="Ahn K" first="Kwang Seok" last="Ahn">Kwang Seok Ahn</name><name sortKey="Baek, Seung Ho" sort="Baek, Seung Ho" uniqKey="Baek S" first="Seung Ho" last="Baek">Seung Ho Baek</name><name sortKey="Kim, Sung Oon" sort="Kim, Sung Oon" uniqKey="Kim S" first="Sung-Hoon" last="Kim">Sung-Hoon Kim</name><name sortKey="Ko, Jeong Yeon" sort="Ko, Jeong Yeon" uniqKey="Ko J" first="Jeong-Hyeon" last="Ko">Jeong-Hyeon Ko</name><name sortKey="Lee, Junhee" sort="Lee, Junhee" uniqKey="Lee J" first="Junhee" last="Lee">Junhee Lee</name><name sortKey="Lee, Seok Geun" sort="Lee, Seok Geun" uniqKey="Lee S" first="Seok Geun" last="Lee">Seok Geun Lee</name><name sortKey="Nam, Dongwoo" sort="Nam, Dongwoo" uniqKey="Nam D" first="Dongwoo" last="Nam">Dongwoo Nam</name><name sortKey="Shim, Bum Sang" sort="Shim, Bum Sang" uniqKey="Shim B" first="Bum Sang" last="Shim">Bum Sang Shim</name><name sortKey="Um, Jae Oung" sort="Um, Jae Oung" uniqKey="Um J" first="Jae-Young" last="Um">Jae-Young Um</name><name sortKey="Yang, Woong Mo" sort="Yang, Woong Mo" uniqKey="Yang W" first="Woong Mo" last="Yang">Woong Mo Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E18 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E18 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:0DEE67EBFC190620F5F6DF24DDF6DAA58D7DD1F6
|texte= Embelin Inhibits Invasion and Migration of MDA‐MB‐231 Breast Cancer Cells by Suppression of CXC Chemokine Receptor 4, Matrix Metalloproteinases‐9/2, and Epithelial–Mesenchymal Transition
}}
| This area was generated with Dilib version V0.6.33. |  |